小鼠E選擇素(SELE)化學發光**分析試劑盒
基本原理:
本試劑盒采用雙抗體夾心法。用抗小鼠SELE抗體包被于酶標板上,實驗時標本或標準品中的SELE會與包被抗體結合,游離的成分被洗去。依次加入生物素化的抗小鼠SELE抗體和辣根過氧化物酶標記的親和素。抗小鼠SELE抗體與結合在包被抗體上的小鼠SELE結合、生物素與親和素特異性結合而形成**復合物,游離的成分被洗去。加入發光底物混合液,發光底物在辣根過氧化物酶的催化下發出熒光,用化學發光**分析儀測定化學發光值(RLU),SELE濃度與化學發光值之間呈正相關,通過繪制標準曲線求出標本中SELE的濃度。
小鼠E選擇素(SELE)化學發光**分析試劑盒描述:
英文名稱 | Mouse SELE (E-Selectin) CLIA Kit | ||
中文名稱 | 小鼠E選擇素(SELE)化學發光**分析試劑盒 | ||
貨號 | KL-M0288c | 種屬 | Mouse/小鼠 |
規格 | 96T/Kit (8*12 wells) | ||
檢測方法 | 雙抗體夾心法 | ||
檢測范圍 | 15.625~1000pg/mL | 靈敏度 | 9.375pg/mL |
小鼠E選擇素(SELE)化學發光**分析試劑盒試劑盒組成:
中文名稱 | 英文名稱 | 規格 | 保存 |
CLIA酶標板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
凍干標準品 | Reference Standard | 2 支 | 4℃/-20℃ # |
標準品&樣品稀釋液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
濃縮生物素化抗體 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
生物素化抗體稀釋液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
濃縮HRP酶結合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
酶結合物稀釋液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
濃縮洗滌液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
發光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
發光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
封板覆膜 | Plate Sealer | 5 張 |
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產品說明書 | Manual | 1 份 |
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質檢報告 | Certificate of Analysis | 1 份 |
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特別說明: #: 一周內使用可存于4℃,需長時間存放或多次使用建議存于-20℃. |
1.在各孔中加入標準品或樣品各 100μL,37℃孵育90分鐘
2. 倒去孔內液體,拍干,加入 100μL 生物素化抗體工作液,37℃孵育 60 分鐘
3. 洗滌 3 次
4. 加入 100μL 酶結合物工作液, 37℃孵育 30 分鐘
5. 洗滌 5 次
6. 加入 100μL 發光底物混合液, 37℃孵育 5 分鐘左右
7. 立即測定各孔的化學發光值
8. 結果計算
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KL-CFB-Eq 馬補體因子B(CFB) 檢測試劑盒 Equine Complement Factor B (CFB) ELISA Kit Equus caballus; Equine Horse 0.156-10 ng/mL 0.07 ng/mL 96T 12 months
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KL-CFB-c 犬補體因子B(CFB) 檢測試劑盒 Canine Complement Factor B (CFB) ELISA Kit Canis familiaris; Canine Dog 78.125-5000 pg/mL 35 pg/mL 96T 12 months
KL-CFB-Ch 雞補體因子B(CFB) 檢測試劑盒 Chicken Complement Factor B (CFB) ELISA Kit Chicken Gallus 46.875-3000 pg/mL 14.6 pg/mL 96T 12 months
KL-CFB-Gu 豚鼠補體因子B(CFB) 檢測試劑盒 Guinea pig Complement Factor B (CFB) ELISA Kit Cavia Guinea pig 31.25-2000 pg/mL 9.8 pg/mL 96T 12 months
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KL-CFB-p 豬補體因子B(CFB) 檢測試劑盒 porcine Complement Factor B (CFB) ELISA Kit Sus scrofa; Porcine Pig 0.156-10 ng/mL 0.05 ng/mL 96T 12 months
KL-CFB-Ra 大鼠補體因子B(CFB) 檢測試劑盒 Rat Complement Factor B (CFB) ELISA Kit Rattus norvegicus Rat 31.25-2000ng/mL 8.8ng/mL 96T 12 months
KL-CFB-Rb 兔補體因子B(CFB) 檢測試劑盒 Rabbit Complement Factor B (CFB) ELISA Kit Oryctolagus cuniculus Rabbit 46.875-3000 pg/mL 11.2 pg/mL 96T 12 months
KL-CFB-Si 猴補體因子B(CFB) 檢測試劑盒 Monkey Complement Factor B (CFB) ELISA Kit Rhesus monkey Simian 62.5-4000 pg/mL 31.2 pg/mL 96T 12 months
KL-ACE-Rb 兔血管緊張素轉化酶(ACE) 檢測試劑盒 Rabbit Angiotensin I Converting Enzyme (ACE) ELISA Kit Oryctolagus cuniculus Rabbit 0.313-20ng/mL 0.108ng/mL 96T 12 months
KL-ACE-b 牛血管緊張素轉化酶(ACE) 檢測試劑盒 Bovine Angiotensin I Converting Enzyme (ACE) ELISA Kit Bos taurus; Bovine Cattle 6.25-400ng/mL 2.15ng/mL 96T 12 months
KL-IL2-Gu 豚鼠白介素2(IL2) 檢測試劑盒 Guinea pig Interleukin 2 (IL2) ELISA Kit Cavia Guinea pig 15.625-1000pg/mL 5.7pg/mL 96T 12 months
KL-CD30L-Ch 雞CD30配體(CD30L) 檢測試劑盒 Chicken Cluster Of Differentiation 30 Ligand (CD30L) ELISA Kit Chicken Gallus 0.156-10 ng/m 0.034 ng/m 96T 12 months
KL-CD40L-b 牛CD40配體(CD40L) 檢測試劑盒 Bovine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit Bos taurus; Bovine Cattle 0.625-40 ng/mL 0.18 ng/mL 96T 12 months